Products Description

Collagen extraction from fish scales：

Pre-treatment: This step involves the removal of impurities such as fat and heteroproteins from the fish skin. For example, anhydrous ether is used as a degreasing reagent and treated for 16 hours at a feed-to-liquid ratio of 1:80 (g/mL); or a 0.6 mol-L^-1 Na2CO3 solution is used to remove heteroproteins by treating the skin twice at 25°C for 6 hours each time.
Extraction: According to different studies, there are various methods for extracting collagen, including alkaline, enzymatic, and ultrasound-assisted enzymatic methods. For example, the optimal conditions for the alkaline extraction of catfish skin collagen for the material-liquid ratio of 1:10, adding 1.6% of Ca (OH) 2, 70 ℃ for 9 hours; enzyme extraction of golden pomfret fish skin collagen for the optimal conditions for the addition of enzyme amount of 1.5%, the liquid-feeding ratio of 23:1 mL / g, the enzyme time of 27.5 hours; ultrasonic-assisted enzyme extraction of mackerel fish skin collagen for the optimal conditions for the The optimal conditions for ultrasonic-assisted enzymatic extraction of mackerel skin collagen were 100 U/g of enzyme, pH 7.5, enzyme digestion temperature of 40 ℃, and ultrasonic treatment time of 50 min.
Purification: the extracted collagen requires further purification to remove possible impurities. For example, dextran gel column chromatography was used to further purify the extracted, salting out and decontaminated collagen extraction from fish scales.
Analysis and characterisation: finally, the extracted and purified collagen was analysed and characterised by various analytical methods (e.g., SDS-PAGE electrophoresis, circular dichroism, time-of-flight mass spectrometry, etc.) to confirm its structure and properties. For example, the results of SDS-PAGE showed that collagen contains α1, α2 and β chains, which is a typical type I collagen; time-of-flight mass spectrometry was used to determine the range of molecular mass distribution of the hydrolysate obtained, and the results showed that its molecular mass was mainly concentrated in the range of 0.6 kDa to 1.8 kDa.