1.The total volume ranges from 10-1000L; the loading factor is ≥ 70%.
2.Stainless steel bioreactor Tank material: The tank is made of 316 stainless steel, and the jacket is made of 304 stainless steel. There are guide plates inside the jacket.
3. The ratio of the diameter to the height of the tank is no more than 1:2.5.
4. Surface treatment: The internal surface of the tank is polished with an accuracy of Ra ≤ 0.4, and is then subjected to surface sandblasting treatment.
5. Pressure Design: Design pressure of the cylinder/cladding is 0.3/0.4 Mpa.
| Parameter | Description |
| Total volume | 5L,10L,15L,20L,30L,50L,100L,200L,500L,1000L, 5KL, 50KL, 200KL etc. |
| Working volume | 30%~70% |
| Tank | Jacket tank, made by SS316 and SS304, tank design pressure 0.3Mpa, jacket design pressure 0.35Mpa, polishing 0.4-0.6um, ratio of diameter and height is 1:2.2-2.5 |
| Stirring system | Mechanical drive on the top/bottom or Magnetic drive on the top, 3pcs height adjustable six-blade paddle Stir speed: 50~1000rpm/50-400rpm |
| Sterilization | Sterilize in place(SIP), auto control by program |
| Clean | Clean in place (CIP) by spray ball |
| Gas control | Control by rotor meter, air flow 1 vvm |
| Feeding | Automatically feeding by peristaltic pump |
| Anti-foam control | Automatically add antifoam by peristaltic pump |
| Temperature control | Auto-control, electric heating range: water source add 5ºC to 70ºC |
| PH control | PH 2.00-12.00±0.1, auto-control adding acid/base by peristaltic pump, pH sensor from Hamilton/Mettler, Switzerland. |
| Do control | 0-150±3% displaying precision 0.1%, DO sensor from Hamilton/Mettler, Switzerland. DO coupling with agitation, oxygen. |
| Sampling | Sampling valve, can sterilization separately, manual |
| Harvest | Zero dead zone bottom valve, can sterilization separately, manual/auto |
Interface settings of the fermentation tank
Tank lid interface
At least ① inoculation port 1; ② feeding port 1; ③ exhaust port 1; ④ pressure sensor interface 1; ⑤ defoaming electrode port 1; ⑥ stirring interface 1; spare port 1; ⑦ supplementary feeding ports 4 (four valve groups)
Upper side interface
At least ① an air intake; ② a strip-shaped mirror opening; ③ a rear-mounted lamp mirror opening
Bottom side interface
At least ① 1 pH electrode interface; ② 1 DO electrode interface; ③ 1 temperature electrode interface; ④ 2 backup electrode interfaces; ⑤ 1 jacket inlet and outlet; ⑥ 1 sampling port
Other parameters
Mixing system
1.Stainless steel bioreactor Equipped with an automatic lifting device, driven by an AC motor for the shaft seal, and a 316L stainless steel stirring shaft.
2. At least 2 layers of 6 flat blades + 1 layer of four inclined blades
3. At least one layer of mechanical defoaming blades, with adjustable blade height and a rotational speed range of ≥ 50 - 1000 rpm ± 5 (customized according to volume), ensuring long-term operation at high speeds.
Temperature control
1.Stainless steel bioreactor adopts electric heating and circulating water cooling. It has the functions of air self-extraction and water cut-off, as well as over-temperature protection. The intelligent PID control ensures the rapidity of temperature control while achieving energy savings.
2. Fermentation temperature control range: Cooling water temperature +5℃ - 65℃, with an accuracy of ≤ 0.2℃ and a resolution of ≤ 0.1℃.
Feed control
1. Controlled by a variable-speed peristaltic pump, featuring 4 adjustable-speed programmable flip-top type stepless speed control peristaltic pumps;
2. Feed accuracy ≤ 0.02 ml/ver;
3. Feed range: 0.02 ml/min - 340 ml/min;
4. Feed methods: constant-speed feeding, exponential feeding (controlled by the growth rate equation), linear feeding, sequential control feeding, feeding associated with pH, feeding associated with dissolved oxygen, etc.
Certification
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