How to optimize the extraction process of collagen from tilapia skin?
By ultrasonic assisted enzymatic hydrolysis,the obtained collagen protein has high yield and good relative solubility, and is widely used in food processing.
Within a certain power range, the cavitation effect produced by ultrasound and
Mechanical effects can modify the structure of the enzyme, expose its active site, and improve the activity of the enzyme. Ultrasound can also change the properties of the substrate, making it easier for collagen to dissolve in the extraction solvent, providing the best conditions for collagen extraction.
Product structure
Ultrasonic assisted extraction is a new extraction method in recent years. The mechanism of its auxiliary extraction of collagen is to use the huge pressure generated by ultrasonic cavitation to make the uncrosslinked collagen molecules separated instantaneously.
The mechanical effects of subsequent ultrasound facilitate separation and increase the rate of collagen extraction, while ultrasound treatment opens up the collagen fibers to facilitate acid and enzyme treatment. Compared with the traditional method, the extraction time can be significantly shortened.
In the process of ultrasonic-assisted extraction, the content of type I collagen in raw materials, ultrasonic time, and ultrasonic per unit area will affect the extraction rate. Therefore, low-frequency high-power ultrasound is often used in industry to assist the extraction of collagen.
Processing flow
Take a certain amount of the above treated fish scale powder, add a certain amount of acetic acid solution and enzymes (as required), placed in the ultrasonic cell crusher extraction. After the end of ultrasound, the conical bottle was placed at a suitable ambient temperature for reaction, and the total time was controlled within 8 hours. After the enzymatic hydrolysis, centrifuge at 8000 r/min for 20 minutes, and retain the supernatant.
