E Coli Bioreactor

E coli bioreactor is a technological system that utilizes engineered Escherichia coli as a host to achieve efficient production of target products through specific culture conditions and strategies. Its advantages include a clear genetic background, ease of handling, rapid reproduction, low growth requirements, low cost, and good safety.

Send Inquiry

Products Description

In bioreactor applications, E. coli can be cultured through different strategies to improve product yield and quality. For example, by optimizing metabolic pathways and expression systems, product yield and quality can be increased while reducing the generation of unwanted by-products.

The main components of e coli bioreactor:

Product Specification

Parameter	Description
Working volume	50%~70%
Tank	Single glass vessel with stainless steel bottom. Lid made by stainless steel 316L with ports, condenser on the cover
Stirring system	Mechanical drive or Magnetic drive(GC type, bottom/top drive), 3 six-blade paddle and 1 foam breaker, each paddle height adjustable. Stir speed: 50~1000rpm/50-500rpm
Sterilization	Off-site sterilization (Autoclave)
Gas control	Control by rotor meter, air flow 1-2vvm.
Feeding	Automatically feeding by peristaltic pump
Anti-foam control	Automatically add antifoamer by peristaltic pump
Temperature control	Auto-control, electric heating range water source add 5℃ to 70℃
PH control	PH 2.00-12.00±0.1, auto-control adding acid/base by peristaltic pump, pH sensor from Hamilton/Mettler, Switzerland.
DO control	0-150±3% displaying precision 0.1%, DO sensor from Hamilton/Mettler, Switzerland. DO coupling with agitation, oxygen.
Sampling	Manual sampling
Control parameters	10 or 15 inch touch screen , Siemens PLC with control parameters (stirring, pH, DO, temperature, feeding, alarm, gas etc.), data analysis, data record, data output, language(RU/EN/CN) and other customized functions
Optional configuration	Remote control, Mass flow meter(auto control gas), O2/N2/CO2, gas(CO2) exhaust analysis, Level sensor, Turbidity(OD) sensor, ORP sensor, weight system, Live cell sensor(Biomass), Printing, Siemens touch screen, etc.,

Advantages

The advantages of e coli bioreactor include the following:

Easy handling and quick assembly
High level of technology and design standards
Ensuring aseptic processes and minimizing batch losses
Suitable for a wide range of cell culture conditions
Supports high-density, aerobic and anaerobic fermentation and protein expression studies